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Image Search Results
Journal: Acta Pharmaceutica Sinica. B
Article Title: Bioengineered miR-328-3p modulates GLUT1-mediated glucose uptake and metabolism to exert synergistic antiproliferative effects with chemotherapeutics
doi: 10.1016/j.apsb.2019.11.001
Figure Lengend Snippet: Production of fully-humanized biologic miR-328-3p molecule. (A) Schematic illustration of the fully-humanized bioengineered RNA agent (hBERA) bearing the target miR-328-3p (hBERA/miR-328, 192 nucleotides in length). The carrier consists of a human serine tRNA (htRNA Ser ) and a modified pre-miR-34a 23 , in which miR-34a duplexes are replaced by target miR-328 sequences. (B) High-level heterogeneous expression of hBERA/miR-328 through bacterial fermentation, as demonstrated by urea-PAGE analysis. Total RNA from wild type (WT) Escherichia coli was used for comparison. (C) FPLC trace during the purification of hBERA/miR-328. The insert shows urea-PAGE analysis of target RNA fractions collected during FPLC separation. (D) The purity of isolated hBERA/miR-328-3p (98.5%) was determined by HPLC analysis. Au/Uv, absorbance unit of ultraviolet-visible spectroscopy; nt, nucleotide.
Article Snippet: Purification of target RNA molecules was achieved by using the
Techniques: Modification, Expressing, Comparison, Purification, Isolation, Spectroscopy